Figure 7.

Immunolocalization of BubR1 in cells lacking Mad2. Control and Mad2-depleted cells where either (A) incubated in MG132 for 2 h or (B) released from the arrest into media containing colchicine (180 min). These cells were fixed and stained to reveal DNA (blue), BubR1 (green), and CID (red). (C) Quantification of the BubR1 kinetochore signal in control and Mad2-depleted cells shows an identical twofold increase in cells released into the colchicine. Note that cells lacking Mad2, similarly to control cells, accumulate BubR1 at kinetochores strongly when released into colchicine. Bar, 5 μm.