Table 1.

Percentage of cells positively cotransfected with GFP and anti-GCP6 shRNAs blindly scored for effects on γ-tubulin and MT distribution

Criteria	Promoter-shRNA						n
	H1	H1-1	H1-2	U6	U6-1	U6-2
Disperse γ-tubulin	2.2 ± 3.1	72 ± 10a	31 ± 23a	3.3 ± 3.3	54 ± 45a	60 ± 35	160
MTs at transnuclear level	0.0 ± 0.0	45 ± 1.3	46 ± 12	0.0 ± 0.0	48 ± 1.6	19 ± 18	101

H1 and U6 alone are empty vectors; -1 or -2 indicate the anti-GCP6 shRNA sequences. Average percentage of cells scoring positive to the morphological criterion with respect to the total EGFP-transfected cells ± SD. Data are averages of three independent experiments, counting n total cells.

^a Statistically significant differences with the averages for cells transfected with the corresponding empty vector (t test, p < 0.05).