Table 2.

Phenotype of CACO-2 stable transfectants expressing C-terminal fragments of GCP6

Criteria	f2 + dox cells	f1 − dox cells	f1 + dox cells
Disperse γ-tubulin (% of cells)	3.4 ± 3.4 (91)	3.0 ± 2.5 (183)	49.5 ± 22 (208)a,b
Decreased apical γ-tubulin (% of cells)	3.6 ± 3.7 (91)	4.5 ± 2.3 (183)	43.9 ± 30 (208)a,b
MTs at transnuclear level (% of cells)	0.4 ± 1.1 (256)	0.0 ± 0.0 (160)	29.2 ± 23 (242)a,b
Decreased apical MTs (% of cells)	5.3 ± 6.6 (256)	4.9 ± 10 (160)	19.0 ± 17 (242)
Centrosomes detached from cortical IFs (as % of total centrosomes)	3.6 ± 3.7 (47)	3.6 ± 3.3 (122)	21.8 ± 14.7 (157)a,b

Effects are blindly scored for γ-tubulin and MT distribution and centrosome localization. The data are presented as average ± SD from four independent experiments (total number of cells counted). The criteria were as follows: disperse γ-tubulin were cells with γ-tubulin signal away from the apicolateral cortical distribution as in Figure 4B, bottom, arrow. Decreased apical γ-tubulin was considered when the average pixel values were <50% of the values in neighboring positive cells (e.g., Figure 7D, arrowheads). MTs at transnuclear level were evaluated in XY confocal images at the lowest extent of the lateral K8 signal, as in Figure 7E, arrows. Decreased apical MTs were evaluated in apical XY confocal images as cells with average pixel values for α-tubulin <50% of the average of neighboring positive cells. Detached centrosomes were verified both in XZ and XY planes to verify that no contact with K8 signal could be found in any plane and expressed as average percent of detached centrosomes ± SD (total number of centrosomes counted).

^a Statistically significant differences with the averages in the first column.

^b Statistically significant differences with the averages in the second column (t test, p < 0.05, n = 6).