Table 1.
Multiple reaction monitoring (MRM) transitions for the liquid chromatography tandem mass spectrometry (ESI-LC-MS/MS) assay of prostanoids, dihydroprostaglandins and isoprostanes.
| Compound | MRM (m/z) | Collision energy (eV) |
|---|---|---|
| PGD1 | 353→317 | 15 |
| PGE1 | 353→317 | 15 |
| PGF1α | 355→311 | 25 |
| 6-keto PGF1α | 369→163 | 23 |
| PGB2 | 333→175 | 20 |
| PGB2-d4 | 337→179 | 20 |
| PGD2 | 351→271 | 17 |
| PGE2 | 351→271 | 17 |
| PGF2α | 353→193 | 25 |
| PGJ2 | 333→271 | 15 |
| Δ12-PGJ2 | 333→271 | 15 |
| 15-deoxy-Δ12,14 PGJ2 | 315→271 | 15 |
| PGD3 | 349→269 | 15 |
| PGE3 | 349→269 | 15 |
| PGF3α | 351→193 | 25 |
| TXB2 | 369→169 | 17 |
| TXB3 | 367→169 | 15 |
| 8-iso-PGE2 | 351→315 | 15 |
| 8-iso-15-keto PGE2 | 349→113 | 23 |
| 8-iso-PGF2α | 353→193 | 25 |
| 8-iso-15-keto PGF2α | 351→315 | 15 |
| 13,14-dihydro PGE1 | 355→337 | 15 |
| 13,14-dihydro-15-keto PGE1 | 353→335 | 12 |
| 13,14-dihydro-PGF1α | 357→113 | 38 |
| 13,14-dihydro-15-keto PGF1α | 355→193 | 32 |
| 13,14-dihydro-15-keto PGE2 | 351→333 | 12 |
| 13,14-dihydro PGF2α | 355→311 | 30 |
| 13,14-dihydro-15-keto PGF2α | 353→113 | 28 |