Fig. 4.

Effects of Hh signaling on growth and differentiation of MM stem cells in vitro. (A) FACS analysis of CD138 expression in NCI-H929 cells treated with vehicle or cyclopamine (5 μM). The percentage of CD138⁺ cells is indicated. (B) Representative FACS analysis of CD19 and CD138 expression in purified MM patient bone marrow cells at initial purification and after 5 days of culture treated with vehicle or cyclopamine (5 μM). (C) Graphical data from five patient samples as described in B. (D) FACS analysis of Hoechst 33342-stained MM cells treated with vehicle or cyclopamine (5 μM). The % side population is indicated in each panel. (E) Clonal expansion assay of CD138^neg and CD138⁺ cells from the NCI-H929 cell line treated with ShhNp (4 nM). Data are shown as mean ± SEM (n = 5).