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. 2007 Feb 27;104(10):3793–3798. doi: 10.1073/pnas.0611733104

Fig. 1.

Fig. 1.

Spatial and temporal analysis of clathrin, AP2, and epsin at CCPs. (A) TIRFM image of a COS-7 cell coexpressing LCa-mRFP (Left) and μ2-GFP (Right). Arrowheads point to individual pits. (B) Select frames from a time series of a single CCP. Merged images show colocalization of LCa-GFP and μ2-GFP throughout the vesicle lifetime. (C) Time course of LCa-mRFP (red) and μ2-GFP (green) fluorescence (fluorescence units, F.U.) at a single CCP. (D) Lifetime distribution of LCa-mRFP (red, n = 100) and μ2-GFP (green, n = 100) spots. (E) TIRFM image of a COS-7 cell coexpressing LCa-mRFP (Left) and epsin-GFP (Right). Arrowheads highlight CCPs. (F) Select frames from a time series of a single CCP. Merged images (Bottom) show colocalization of clathrin and epsin throughout the vesicle lifetime. [Scale bars, 2 μm (A and E); 1 μm (B and F).]