Fig. 2.

DgrA is a member of a new family of c-di-GMP-binding proteins. (A) UV cross-linking of purified His₆-tagged diguanylate receptor proteins with c-[³³P]di-GMP. The following proteins were used: DgrA (CC1599; C. crescentus), DgrB (CC3165; C. crescentus), PA4608 (P. aeruginosa), YcgR (S. typhimurium), and BSA (control). The Coomassie blue-stained gel (Left) and the autoradiograph (Right) are shown. (B) UV cross-linking of 10 μM DgrA in the presence of 60 nM ³³P-labeled c-di-GMP. Autoradiograph (Upper) and Coomassie-stained gel (Lower). (Left) Samples were supplemented with increasing concentrations of nonlabeled nucleotides as indicated. (Right) Controls were carried out in the absence of UV irradiation or with BSA.