Fig. 5.

C-di-GMP binding and motility control of DgrA mutants. (A) UV cross-linking of different DgrA mutant proteins with c-[³³P]di-GMP. Coomassie blue-stained SDS/PAGE (Upper) and autoradiograph (Lower) with purified wild-type and mutant DgrA proteins (10 μM) are shown. (B) Motility behavior of C. crescentus wild-type CB15 overexpressing different dgrA alleles: CB15/pBBR::dgrA (a), CB15/pBBR::dgrAR11AR12A (b), CB15/pBBR::dgrAD38A (c), CB15/pBBR::dgrAV74A (d), CB15/pBBR::dgrAR11AR12AV74A (e), CB15/pBBR::dgrAW75A (f), and CB15/pBBR (vector control) (g). Three different colonies from independent conjugation experiments are shown.