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Sequence alignment of the c-di-GMP-binding proteins DgrA, DgrB, YcgR, and PA4608 according to the PilZ Pfam entry PF07238. The PilZ domain is highlighted in green. DgrA residues shown to be important for c-di-GMP binding and in vivo function (red) and the positions of intragenic dms suppressor mutations (black) are highlighted above the alignment. Residues of PA4608 with large chemical shift differences upon c-di-GMP binding (blue) are indicated below the alignment.
