Fig. 4.

Renal ultrastructural changes in homozygous RAG2 KO; hum-X VEGF KI double-homozygous mice treated with anti-VEGF-A antibodies (murine Fc framework). (A and B) Treatment with G6–31 5 mg/kg, weekly for 8 weeks, results in diffusely increased mesangial cellularity, widening of mesangium and capillary loops by amorphous extracellular material (1-μm methacrylate sections; Jones silver stain (A) and toluidine blue (B). (C and D) Treatment with G6–31 10 mg/kg, twice weekly for 8 weeks, results in variably distorted EC, focally enveloping amorphous subendothelial deposits (asterisks indicate presumably immune complexes); EC frequently lack regularly spaced fenestrations. Basement membrane shows focal reduplication (arrows). Podocyte foot processes are variably fused.