Fig. 3.

siRNA Knockdown of c-Src protein levels inhibits DENV infection. Huh-7 cells were transfected with different concentrations of a c-Src-specific siRNA pool or control siRNA. (A) Knockdown of c-Src protein expression was confirmed by Western blot analysis by using a c-Src specific antibody. Lane 1, Control (siGlo RNA-300 nM); lane 2, c-Src specific siRNA pool–200 nM; lane 3, c-Src specific siRNA pool–300 nM. Detection of GAPDH was included as a well loading control. (B) DENV2 infection was reduced by >1.5-log units in cells transfected with 200 nM of the c-Src siRNA pool but was unchanged in the presence of 200 nM siGlo or 200 nM GAPDH SMARTpool negative control reagents.