Fig. 3.

Effect of androgen receptor deficiency on heat shock protein 90α (Hsp90α) and mRNA levels in 15.5-dpc testes. (A) immunohistochemical staining of Hsp90α in control and testicular feminized (Tfm) testes. (Scale bars, 10 μm.) (B and C) Oct-4 (B) and Hsp90α (C) mRNA expression in 15.5-dpc testis from control and Tfm fetuses was measured by Q-PCR using β-actin or Oct-4, respectively, as reference. Results are expressed in relative units with the control animals having a value of 1. n = 5; ∗, P < 0.05; ∗∗, P < 0.01 in Student's t test.