Fig. 5.

Cell-specific expression of androgen receptor protein in 15.5-dpc testis. (A) Immunohistochemical staining of androgen receptor in control and testicular feminized (Tfm) testes. The arrowhead indicates an example of a stained gonocyte. No signal was detected in Tfm testes. (Scale bars, 10 μm.) (B) Enriched gonocyte cultures were transfected with the ARE₂-TATA-GFP-NLS vector and cultivated with or without dihydrotestosterone (DHT) (10⁻⁶ M) for 30 h. GCNA1, a gonocyte marker, and GFP were detected by immunostaining (red and green, respectively). Green gonocytes were present only after DHT treatment indicating the presence of a functional androgen receptor protein. (C) Five-day-old Sertoli cell cultures served as positive control. Cells were transfected with ARE₂-TATA-GFP-NLS vector and cultivated without or with DHT (10⁻⁶ M). Anti-mullerian hormone, a cytoplasmic marker of Sertoli cells, and GFP were detected by immunostaining (red and green, respectively). As expected, green Sertoli cells were present only after DHT treatment indicating the presence of a functional androgen receptor protein. All of the slides in B and C were counterstained with 4′-6 diaminido-2-phenylindole (DAPI) to visualize nuclei (blue). (Scale bars, 10 μm.)