Fig. 1.

Dwell events obtained from single-molecule experiments with myosin V and myosin VI. (a) Dwells in mechanical stepping of a double-headed myosin VI. (b) Dwells of actin binding and unbinding for single-headed myosin V. The portions of large oscillations correspond to the actin unbound states, whereas the portions of small fluctuations correspond to the actin bound states. (c) The movement of an actin filament (top arrow) driven by the power stroke of a single-headed myosin V attached to a bead. The arrows on the left of myosin indicate the cyclic behavior of binding, power stroke, unbinding, and recovery.