Fig. 1.

The vti12 mutant secretes VAC2 and BL. (A) Shown are plants homozygous for the VAC2 construct, derived from crosses of L1 with the wild type (Wt) ecotype Col-0, with vti12 and with vti11 plants. The genotype of the plants is shown above the figures. (B) The presence of BL in the apoplastic fluid (Apo) from Wt, vti12, and vti11 mutant lines homozygous for the BL transgene was analyzed by Western blot with anti-BL antibodies. Silver staining of the samples is shown as loading control. The accumulation of BL in a total fraction (Tot) from Wt is shown for comparison.