Fig. 3.

VTI12 is required for trafficking of GFP-CHI to the vacuole. Leaves from plants with designated genotypes were transiently transformed with GFP-CHI and ALEU-GFP constructs. GFP fluorescence was observed by CLSM. Transformed cells shown are representative of the patterns observed in the various genotypes. Arrows indicate cells showing weak diffuse vacuolar fluorescence and accumulation of small vacuoles; arrowheads indicate speckled structures accumulating ALEU-GFP; Wt, Col-0. (Scale bar: 10 μm.)