Fig. 1.

Screening the proteome arrays with an RNA containing a CAM. (A) The sequence of the RNA SLD4 containing the CAM motif is shown along with a structure of the CAM (12). The clamped adenine is residue eight in SLD4 and a substitution to a guanine that abolishes CAM formation in RNA mSLD4 is shown below SLD4. Other nucleotides in mSLD4 are identical to those in SLD4. (B) A scheme illustrating the probing of the protein array. An equal mixture of SLD4 with a 5′ Cy3, and mSLD4 with a 5′ Cy5 was used as the probe. Images of the protein spots of two of the positive hits, Pus4 and App1, and the internal positive control, the BMV CP, are shown below the array. (C) The distribution of the results (the median of Cy3/Cy5 ratios) from the array screen. The signal of BMV CP protein is identified within the graph.