Table 1.

Germ-line transformation in flies transgenic for attP and various φC31 integrase constructs

Line	Integrase construct	DNA injected pUAS-lacZattB, ng/μl	Giving w+ progeny
			w+/total no.	%
Set-up I
nos-φ-zh86Fb/attP-zh102D	nos-φC31-nos	215	28/70	40
nos-φNLS-zh86Fb/attP-zh102D	nos-φC31NLS-nos	215	52/122	43
vas-φ-zh86Fb/attP-zh102D	vas-φC31-vas	215	29/69	42
vas-φNLS-zh86Fb/attP-zh102D	vas-φC31NLS-vas	215	25/77	32
vas-φ-zh86Fb/attP-zh102D	vas-φC31-vas	800	32/80	40
Set-up II
attP-zh86Fb/nos-φ-zh102D	nos-φC31-nos	215	16/103	16
attP-zh86Fb/nos-φNLS-zh102D	nos-φC31NLS-nos	215	49/148	33
attP-zh86Fb/vas-φ-zh102D	vas-φC31-vas	215	47/93	51
attP-zh86Fb/vas-φNLS-zh102D	vas-φC31NLS-vas	215	16/49	33
attP-zh86Fb/vas-φ-zh102D	vas-φC31-vas	800	36/66	55

The lines injected were homozygous for both an attP site and a φ C31 integrase construct. G₀ adults (male and females) obtained after injection were individually crossed with y w animals and progeny were screened for white⁺ expression. Line designations indicate the origin of the integrase constructs and the free attP site (e.g., nos-φ-zh86Fb/attP-zh102D:nos-φC31 construct is located in the attP site of line ZH-attP-86Fb, and the free attP site is derived from line ZH-attP-102D). Because single G₀ females generally exhibited a low fertility and transgenesis rate compared with males, in all subsequent transformation experiments two injected females were used for one y w outcross. However, although we still tested females for transgenic frequencies, we present in the Tables 2 and 3 only the transgenic values obtained from G₀ males. The percentages indicate the fraction of all the fertile G₀ adults that gave white⁺ offspring.