Figure 5.

Treatment of t(1;19)-positive pre-B acute lymphoblastoid cell lines with antisense 2′-methoxyethyl phosphorothioate oligonucleotides to E2A-Pbx1 reveals that WNT-16 transcript levels are dependent on the presence of E2A-Pbx1. 697 cells were electroporated with antisense oligonucleotides with specificity for E2A-Pbx1. Twenty hours later, total RNA was derived from the cells and analyzed by Northern blotting by using asymmetric PCR probes for E2A-Pbx1 and WNT-16 and a random primer-labeled cDNA probe for GAPDH. (A) Northern blots. Nalm-6 (N-6) RNA was included as a control. (B) Graphical representation of Northern blot results. Relative mRNA levels detected on Northern blots were quantitated by using a PhosphorImager. Bar = mean ± SD from three independent experiments.