Skip to main content
. 2007 Feb 21;104(9):3153–3158. doi: 10.1073/pnas.0609103104

Fig. 2.

Fig. 2.

Large-scale interdomain motion of the A and B domains in phosphoIIBAMtl ascertained from RDC measurements. Shown is a comparison of observed and calculated 1DNH RDCs measured in a 5% neutral polyacrylamide gel for the B (a) and A (b) domains of phosphoIIBAMtl. The magnitude of the axial component of the alignment tensor (Da) and the rhombicity (η) are −8.2 Hz and 0.35 for the B domain but −4.2 Hz and 0.43 for the A domain, respectively. The RDC R factors and correlation coefficients are 12.4% and 0.99 for the B domain and 20.2% and 0.96 for the A domain, respectively. If the RDC data for the A and B domains are fit simultaneously to the coordinates of the IIAMtl–phosphoIIBMtl complex, the RDC R factor is increased to 58.4%. The coordinates of the A and B domains are taken from the coordinates of the IIAMtl–phosphoIIBMtl complex (ref. 12; PDB ID code 2FEW).