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. 2007 Feb 21;104(9):3438–3443. doi: 10.1073/pnas.0611699104

Fig. 1.

Fig. 1.

LRP is necessary for tat-induced apoptosis. (A) Schematic illustrating cocultures, with neurons growing on top of astrocytes; neurons were imaged in a more superficial optical section. (B–D) Double staining for TUNEL (apoptosis) and MAP-2 (neurons). (E–G) Staining for TUNEL and GFAP (astrocytes). There was minimal apoptosis in untreated cultures (B and E), increased apoptosis after 24 h of tat treatment (C and F), and reduced apoptosis when the LRP inhibitor, RAP, was applied 15 min before and 6 h after tat (D and G). (H and I) Percentage of TUNEL-positive cells after different durations of treatment with tat alone (●) or with tat plus RAP. Control cells without treatment did not show increased apoptosis (data not shown). Addition of RAP once (15 min before tat treatment, RAP, ■), twice (a second application 6 h after tat treatment, RAPX2, ▴), or three times (at 6 and 12 h after tat treatment, RAPX3, ▾) reduced tat-induced apoptosis in neurons and astrocytes (P < 0.05 for all treatments vs. control, n = 7, no significant difference between RAP treatments).