Table 1.
Suppression efficiency of M. barkeri tRNAPyl variants
| Mutations | Suppression efficiency, % |
|---|---|
| Wild type | |
| Fusaro | 100 |
| MS (C44U/A3G) | 95 |
| Discriminator base | |
| G73→C | 88 |
| G73→A | 33 |
| G73→U | 43 |
| Acceptor stem | |
| G1→A | 26 |
| C72→U | 30 |
| G1:C72→C:G | 70 |
| G2:C71→A:U | 52 |
| A3→G | 58 |
| A5:U68→C:G | 100 |
| D-stem and loop | |
| U15→A | 39 |
| G18→U | 87 |
| U20→A | 43 |
| A21→U | 95 |
| G10:C25→U:A | 50 |
| A11:U24→C:G | 88 |
| U12:A23→G:C | 112 |
| C13:G22→A:U | 25 |
| Insert at G18 | 47 |
| TψC-stem and loop | |
| U54→A | 80 |
| A56→U | 33 |
| A58→U | 40 |
| U60→A | 108 |
| C50:G64→A:U | 79 |
| G51:C63→U:A | 29 |
| G52:C62→U:A | 63 |
| G53:C61→U:A | 63 |
| Variable loop | |
| C44→U | 56 |
| A45→G | 23 |
| G48→U | 17 |
| Insert A at C44 | 17 |
| Anticodon stem/loop | |
| A27:U43→G:A | 56 |
| U29a→C | 40 |
| Δ(U29aG41b) | 12 |
| G29b:C41a→U:A | 80 |
| G30:C40→ U:A | 74 |
| A31:U39→C:G | 46 |
| C32→A | 31 |
| U33→G | 16 |
| A37→C | 13 |
| A38→C | 49 |
Suppression efficiency was measured by β-galactosidase activity in E. coli strain XAC/A24. A percentage of 100 corresponds to 3,800 Miller units (13). In the absence of Cyc, background suppression in all cases was <5%.