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Suppression efficiency of tRNASer/Pyl chimeras
| tRNA | Suppression efficiency, % |
|---|---|
| tRNACUASer | <5 |
| Stabilized tRNACUASer | <5 |
| tRNASer/Pyl chimera 1 | 62 |
| tRNASer/Pyl chimera 2 | 69 |
| tRNAPyl | 100 |
Suppression efficiency was measured by β-galactosidase activity in E. coli strain XAC/A24. In the absence of Cyc, background suppression in all cases was <5%.
