Fig. 4.
The efficiency of X4-tropic HIV infection in HOS cells transfectants by using HIV-1NL4–3 strain (A), S.I. clinical isolates (B), and N.S.I. clinical isolates (C). S.I. represents an isolate with CXCR4 predominance, and N.S.I. represents and isolate with CCR5 predominance. Red, blue, and black lines show the means from three three-replicate assays for CXCR1-Ha (red lines), CXCR1-HA (blue lines) and control cells transfected with empty vector (black lines). Vertical bars indicate the range of the results obtained for each set of measurements. The amount of p24 protein in culture medium is indicated as ng/ml. Differences between CXCR1-Ha and CXCR1-HA for day 8 are significant in all instances as evaluated by a t test: NL, P = 0.002; S.I.-1, P = 0.001; S.I.-2, P = 0.01; S.I.3, P = 0.008; N.S.I.-1, P = 0.003; N.S.I.-2, P = 0.006; N.S.I.-3, P = 0.03.