Table 2.
Apparent kinetic constants for S. enterica serovar Typhimurium RmlC mutants with dTDP-6-deoxy-D-xylo-4-hexulose.
| Enzyme | Km (mM) | kcat (1/s) |
|---|---|---|
| Native | 0.71 ± 0.17 | 39 ± 6.6 |
| RmlC K73A | 0.35 ± 0.051 | 0.095 ± 0.0083 |
| RmlC Y133F | 0.48 ± 0.21 | 0.016 ± 0.0017 |
| RmlC H63A | N.D | 0 |