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. Author manuscript; available in PMC: 2008 Jan 5.
Published in final edited form as: J Mol Biol. 2006 Sep 29;365(1):146–159. doi: 10.1016/j.jmb.2006.09.063

Table 3.

Isothermal titration calorimetry data for P. aeruginosa RmlC with sugar nucleotides. The uncertainties are estimated from the fitting and underestimate the true error.

Ka (× 103M) ΔH (kJmol−1)
dTDP-L-rhamnose 11.6 ± 1.8 −8.6 ± 0.9
dTDPa 13.2 ± 1.7 −25 ± 2
dTDP-xylose 1.32 ± 0.05 −11.8 ± 0.2
dTDP-glucose 0.96 ± 0.26 −9.3 ± 6.2
a

The values for dTDP did vary depending on experiment and on the processing parameters. As is seen for dTDP-L-rhamnose (Fig 5) there is an endothermic event at the start of titration that we cannot interpret. If the endothermic points are omitted, N is set to 1 and the values recalculated the Ka drops to 4.5 × 103M. Our interpretation is that dTDP binds more tightly than dTDP-xylose/glucose, we do not think our data robust enough to compare dTDP and dTDP-L-rhamnose.