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. Author manuscript; available in PMC: 2007 Feb 28.
Published in final edited form as: Free Radic Biol Med. 2006 Oct 28;42(2):299–310. doi: 10.1016/j.freeradbiomed.2006.10.049

Figure 5.

Figure 5

Obese mice are more susceptible to UVB-induced phosphorylation of MAPK protein than wild-type mice. Chronic exposure of mice to UVB induces phosphorylation of ERK1/2 (A), JNK (B) and p38 (C) proteins of MAPK family, and induces inactivation of ERK1/2, JNK and p38 MAP kinase phosphatases (D). Mice were treated as described in Figure 2. Skin lysates were prepared to determine the phosphorylated and total protein levels of ERK1/2, JNK and p38, and the levels of ERK, JNK and p38 MAP kinase phosphatases using western blot analysis. A representative blot is shown from three independent experiments with identical observations, and equivalent protein loading was confirmed by probing stripped blots for β-actin as shown. The relative density of each band in pMAPK immunoblots are shown in terms of the ratio of the band densities of respective MAPK immunoblots. The densitometry value in case of total MAPK protein was assigned the value 1 (arbitrary unit).