Table 1.
A | B | C | D | E | F | G | H | I | J | K | |
---|---|---|---|---|---|---|---|---|---|---|---|
NaCl | 150 | — | 150 | — | — | 75 | 140 | 140 | 20 | 20 | 10 |
ChCl | — | 120 | — | — | — | — | — | — | — | — | — |
NH4Cl | — | — | — | 150 | — | — | 1 | 10 | — | — | — |
CsCl | — | — | — | — | 150 | — | — | — | — | — | — |
NaHCO3 | — | — | — | — | — | — | — | — | 22 | 2.2 | 22 |
CaCl2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
MgCl2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Glucose | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
Hepes-Tris | — | — | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
pH | 7.4 | 7.4 | 7.4 | 7.4 | 7.4 | 7.4 | 7.4 | 6.4 | 7.4 | 6.4 | 7.4 |
O2 (%) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 95 | 95 | 95 |
CO2 (%) | — | — | — | — | — | — | — | — | 5 | 5 | 5 |
Solute concentrations are mm. All solutions were adjusted with mannitol to an osmolarity of ∼320 mosmol l−1. ChCl, choline chloride.