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. 2007 Feb 28;104(10):3693–3697. doi: 10.1073/pnas.0611713104

Fig. 3.

Fig. 3.

Orthogonal staining of HEK293T cells expressing two proteins on the PM. (A) Connexin43–4C with an intracellular tetracysteine motif, stained with ReAsH (ex568/55, em653/95). (B) His6 on extracellular PM surface stained and visualized with HisZiFiT (ex495/10, em535/45). (C) Merge of images A and B representing HisZiFiT staining of His6-CFP-GPI (green) and ReAsH staining of Connexin43–4C (red).