TABLE 2.
Strains used to measure homeologous recombination using the HIS3::intron recombination assay
| Strain | Genotype | Recombination substrates |
|---|---|---|
| SJR769 | wild-type | cβ2/cβ2: homologous substrates |
| SJR770 | wild-type | cβ2/cβ7: predicted to form 9% base-base mismatches |
| GCY615 | wild-type | cβ2/cβ2-ns: predicted to form four (1.3%) base-base mismatches |
| SJR767 | wild-type | cβ2/cβ2-1L: predicted to form four 1-nt loops |
| GCY559 | wild-type | cβ2/cβ2-4L: predicted to form four 4-nt loops |
| SJR768 | wild-type | cβ2/cβ2-12L: predicted to form two 12-nt loops |
| EAY1605 | msh2Δ | cβ2/cβ2 |
| EAY1607 | msh2Δ | cβ2/cβ7 |
| EAY1609 | msh2Δ | cβ2/cβ2-ns |
| EAY1611 | msh2Δ | cβ2/cβ2-1L |
| EAY1613 | msh2Δ | cβ2/cβ2-4L |
| EAY1615 | msh2Δ | cβ2/cβ2-12L |
| EAY1617 | msh3Δ | cβ2/cβ2 |
| EAY1619 | msh3Δ | cβ2/cβ7 |
| EAY1621 | msh3Δ | cβ2/cβ2-ns |
| EAY1623 | msh3Δ | cβ2/cβ2-1L |
| EAY1625 | msh3Δ | cβ2/cβ2-4L |
| EAY1627 | msh3Δ | cβ2/cβ2-12L |
| EAY1629 | msh6Δ | cβ2/cβ2 |
| EAY1631 | msh6Δ | cβ2/cβ7 |
| EAY1633 | msh6Δ | cβ2/cβ2-ns |
| EAY1635 | msh6Δ | cβ2/cβ2-1L |
| EAY1637 | msh6Δ | cβ2/cβ2-4L |
| EAY1639 | msh6Δ | cβ2/cβ2-12L |
| EAY1641 | msh2Δ1 | cβ2/cβ2 |
| EAY1643 | msh2Δ1 | cβ2/cβ7 |
| EAY1645 | msh2Δ1 | cβ2/cβ2-ns |
| EAY1647 | msh2Δ1 | cβ2/cβ2-1L |
| EAY1649 | msh2Δ1 | cβ2/cβ2-4L |
| EAY1651 | msh2Δ1 | cβ2/cβ2-12L |
| EAY1653 | msh2Δ1 msh3Δ | cβ2/cβ2 |
| EAY1655 | msh2Δ1 msh3Δ | cβ2/cβ7 |
| EAY1657 | msh2Δ1 msh3Δ | cβ2/cβ2-ns |
| EAY1659 | msh2Δ1 msh3Δ | cβ2/cβ2-1L |
| EAY1661 | msh2Δ1 msh6Δ | cβ2/cβ2 |
| EAY1663 | msh2Δ1 msh6Δ | cβ2/cβ7 |
| EAY1665 | msh2Δ1 msh6Δ | cβ2/cβ2-ns |
| EAY1667 | msh2Δ1 msh6Δ | cβ2/cβ2-1L |
The SJR769, SJR770, SJR767, GCY559, GCY615 strains bearing the above recombination substrates are described.20 The mismatches predicted to form in heteroduplex DNA during genetic recombination are indicated for each substrate. Integration vectors used to make the indicated msh strains were: pEAI98 (msh2Δ::hisG-URA3-hisG), pEAI88 (msh3Δ::hisG-URA3-hisG), pEAI108 (msh6Δ::hisG-URA3-hisG), and pEAI244 (msh2Δ1::KanMX).16, 31 Note that the intact hisG-URA3-hisG cassette is present in these msh2Δ, msh3Δ and msh6Δ mutant derivatives.