TABLE 4.
substrate | genotype | rate of His+ recombinants (X 10−6) | normalized homeologous rate** | mutant/wild-type*** |
---|---|---|---|---|
Cβ2/Cβ2 (homologous) | wild-type | 2.7 (2.5–3.8)* | ||
msh2Δ | 5.5 (4.7–9.2) | |||
msh3Δ | 6.6 (6.2–9.6) | |||
msh2Δ1 | 7.0 (5.5–8.8) | |||
msh6Δ | 2.0 (1.3–2.4) | |||
msh2Δ1 msh3Δ | 9.6 (9.0–11) | |||
msh2Δ1 msh6Δ | 10 (8.9–12) | |||
Cβ2/Cβ7 (9% diverged) | wild-type | 0.087 (0.057–0.11) | 0.032 | 1.0 |
msh2Δ | 4.4 (3.8–7.2) | 0.80 | 25 | |
msh3Δ | 0.61 (0.5–0.79) | 0.093 | 2.9 | |
msh2Δ1 | 0.51 (0.48–0.71) | 0.073 | 2.3 | |
msh6Δ | 0.40 (0.36–0.44) | 0.20 | 6.4 | |
msh2Δ1 msh3Δ | 1.1 (0.89–1.6) | 0.11 | 3.4 | |
msh2Δ1 msh6Δ | 7.1 (5.5–8.4) | 0.70 | 22 | |
Cβ2/Cβ2-ns (1.3% diverged) | wild-type | 0.16 (0.15–0.22) | 0.060 | 1.0 |
msh2Δ | 8.7 (5.2–10) | 1.6 | 26 | |
msh3Δ | 1.8 (1.2–2.4) | 0.28 | 4.6 | |
msh2Δ1 | 1.8 (1.5–2.8) | 0.26 | 4.3 | |
msh6Δ | 2.2 (1.9–2.4) | 1.1 | 19 | |
msh2Δ1 msh3Δ | 4.9 (4.3–6.1) | 0.51 | 8.5 | |
msh2Δ1 msh6Δ | 16 (12–17) | 1.5 | 26 | |
Cβ2/Cβ2-1L | wild-type | 0.29 (0.25–0.49) | 0.11 | 1.0 |
msh2Δ | 11 (9.6–15) | 2.1 | 19 | |
msh3Δ | 3.6 (2.5–4.8) | 0.54 | 5 | |
msh2Δ1 | 4.3 (2.8–5.6) | 0.61 | 5.6 | |
msh6Δ | 0.63 (0.48–0.83) | 0.32 | 3 | |
msh2Δ1 msh3Δ | 6.2 (6.0–7.7) | 0.64 | 6 | |
msh2Δ1 msh6Δ | 21 (16–24) | 2.1 | 19 | |
Cβ2/Cβ2-4L | wild-type | 0.25 (0.15–0.30) | 0.091 | 1.0 |
msh2Δ | 6.7 (6.5–8.4) | 1.2 | 13 | |
msh3Δ | 7.6 (6.6–9.1) | 1.2 | 13 | |
msh2Δ1 | 7.4 (4.7–7.7) | 1.1 | 12 | |
msh6Δ | 0.27 (0.21–0.34) | 0.14 | 1.5 | |
Cβ2/Cβ2-12L | wild-type | 0.49 (0.40–0.60) | 0.18 | 1.0 |
msh2Δ | 5.7 (3.8–6.6) | 1.0 | 5.7 | |
msh3Δ | 5.7 (5.1–10) | 0.86 | 4.8 | |
msh2Δ1 | 6.2 (4.9–7.4) | 0.88 | 4.9 | |
msh6Δ | 0.51 (0.42–0.74) | 0.26 | 1.4 |
Numbers in parentheses indicate 95% confidence intervals. Homeologous recombination rates and 95% confidence intervals were calculated as described in the Materials and Methods from 14–21 individual cultures. The genotype of the strains is shown in Table 2.
homeologous rate normalized to homologous rate in strain containing the same msh genotype.
mutant homeologous rate relative to wild-type homeologous rate.20