TABLE 5.
Rates of microsatellite instability in strains bearing small deletion and point mutations in Domains I and IV of MSH2, MSH3, and MSH6
| nt in repeat unit | genotype | avg. rate of tract alteration (X10−6) | relative rate |
|---|---|---|---|
| 1 | wild-type | 4.2 (3.2–6.2) | 1.0 |
| msh2Δ | 20,000 (14,000–23,000) | 4,800 | |
| msh3Δ | 740 (560–1,200) | 180 | |
| msh2Δ1 | 760 (500–1,500) | 190 | |
| msh2Δ1a | 760 (520–1,000) | 190 | |
| msh2Δ1b | 15,000 (11,000–35,000) | 3,600 | |
| msh2Δ1c | 20,000 (19,000–33,000) | 4,800 | |
| msh2F19A | 15 (6.8–21) | 3.5 | |
| msh2Y42A | 4.3 (2.8–8.3) | 1.0 | |
| msh2Q89A | 8.9 (5.7–20) | 2.1 | |
| msh2I108A | 15 (10–30) | 3.5 | |
| msh3Δ | 740 (560–1,200) | 180 | |
| msh3Δ1 | 780 (470–1,400) | 190 | |
| msh3Δ1a | 770 (710–1,700) | 180 | |
| msh3Δ1b | 420 (340–820) | 100 | |
| msh3P163A | 3.5 (2.2–6.1) | 0.83 | |
| msh3G185A | 7.5 (4.2–9.3) | 1.8 | |
| msh3K187A | 17 (12–25) | 4.1 | |
| msh3K189A | 38 (27–100) | 9.0 | |
| msh3K187A,K189A | 320 (260–420) | 76 | |
| msh3P232A | 13 (5.3–22) | 3.1 | |
| msh3Q255A | 8.3 (2.0–14) | 2.0 | |
| msh3R276A | 400 (250–730) | 95 | |
| msh3K701A | 3.4 (2.8–5.2) | 0.81 | |
| msh3R705A | 3.8 (2.5–7.9) | 0.90 | |
| msh6Δ | 83 (46–120) | 20 | |
| msh6F337A | 69 (25–80) | 16 | |
| 2 | wild-type | 1.5 (0.7–2.0) | 1.0 |
| msh2Δ | 540 (420–890) | 360 | |
| msh2Δ1 | 85 (52–140) | 57 | |
| msh3Δ | 79 (37–120) | 53 | |
| msh3K187A | 10 (5.5–20) | 7.0 | |
| msh3K189A | 22 (10–34) | 15 | |
| msh3K187A,K189A | 80 (72–100) | 53 | |
| msh3R276A | 44 (39–62) | 29 | |
| 4 | wild-type | 2.1 (1.6–3.0) | 1.0 |
| msh2Δ | 120 (74–170) | 57 | |
| msh2Δ1 | 110 (75–130) | 53 | |
| msh3Δ | 130 (72–160) | 62 | |
| msh3K187A | 7.5 (3.2–14) | 3.6 | |
| msh3K189A | 31 (16–93) | 15 | |
| msh3K187A,K189A | 140 (110–190) | 67 | |
| msh3R276A | 74 (47–110) | 35 |
*
Numbers in parentheses indicate 95% confidence intervals. Microsatellite instability rates and 95% confidence intervals were calculated as described in the Materials and Methods. The indicated strains were transformed with the reporter plasmids containing the indicated repeat unit (Table 1).