Figure 5. Acute and chronic CTZ modulation of mEPSCs in hippocampal cultures.

A–C, typical mEPSC traces recorded in the presence of TTX (0.5 μm) and BIC (20 μm) in control (A), acute application (B), and after chronic pretreatment (C) with CTZ (both 5 μm). Insets at the bottom of each panel are the averaged mEPSCs, showing a slowed decay phase after acute application of CTZ (B) but not chronic CTZ-pretreatment (C, after washout of CTZ). D, bar graphs showing that the normalized mEPSC frequency was significantly increased to 147 ± 11% during acute CTZ application (P < 0.02), and to 193 ± 35% after chronic CTZ pretreatment (P < 0.05). E, bar graphs showing that the normalized mEPSC amplitude was not significantly changed during acute application (107 ± 6%, P > 0.3) or after chronic pretreatment with CTZ (119 ± 12%, P > 0.05). F, kinetics analysis showing a significant increase in the normalized decay time constant during acute application of 5 μm CTZ (156 ± 12%, P < 0.003), but not after chronic pretreatment with CTZ (91 ± 10%, P > 0.5).