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. 1999 Sep 28;96(20):11476–11481. doi: 10.1073/pnas.96.20.11476

Figure 4.

Figure 4

Effect of CTLA-4 blockade on the response of tumor-bearing mice to delayed immunization. Tumor-bearing mice or tumor-free mice received anti-HA TCR+ transgenic T cells on day 0 and were treated with anti-CTLA-4 antibodies or hamster IgG antibodies (days −1, 0, +1) or were left untreated as in Fig. 3. vacc-HA immunization was delayed until day +6 and the analysis was performed 6 days later. (A) T cells from unimmunized mice (solid bars), vacc-HA-immunized mice (interrupted cross-hatched bars), and mice treated with anti-CTLA-4 antibodies plus vacc-HA (hatched bars) were analyzed by FACScan. Values represent mean + SE of percentage of T cells expressing the clonotypic TCR for three mice per group. T cells from the mice in A were analyzed for their in vitro proliferation (B) as well as IFN-γ production (C) in response to stimulation with HA peptide. Values are the mean + SE of triplicate cultures from three mice in each group. (D) Proliferative response to stimulation with splenocytes infected with vaccinia virus was determined as in Fig. 1.