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. Author manuscript; available in PMC: 2008 Mar 16.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Jan 18;354(3):757–763. doi: 10.1016/j.bbrc.2007.01.050

Fig 3.

Fig 3

Effect of SnMP on Bach1 expression. (A) Cells were incubated with SnMP (0, 1, 10, and 20 μM) for 24h, harvested, and then cytosolic and nuclear fractions were extracted. Cytosolic and nuclear fractions were run on SDS/PAGE and immunoblotted with Bach1 antibodies. Coomassie blue staining confirmed equal loading of protein in each lane. (B) Time-dependent effect of SnMP on HO-1 (●), HO-2 (□), and Bach1 (◆) protein expression. Cells were treated with vehicle or 20-μM SnMP for indicated time periods. Cytosolic and nuclear fractions were extracted and then run on SDS/PAGE and immunoblotted with HO-1, HO-2, and Bach1 antibodies. *P<0.01, **P<0.0001 vs. control. Data are shown as mean±SD of 3 observations. (C) Transcriptional regulation of Bach1 and protein stability by SnMP. NIH3T3-HO-1-luc cells were treated with 10-μg CHX/ml (◆) alone or with 20-μM SnMP (□) and harvested at the indicated time points for nuclear and cytosolic protein isolation. Nuclear extracts were run on SDS/PAGE and immunoblotted with Bach1 antibody. Coomassie blue staining confirmed equal loading of samples. Data are representative of two independent experiments.