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. Author manuscript; available in PMC: 2007 Mar 1.
Published in final edited form as: J Mol Biol. 2005 Sep 2;351(5):948–955. doi: 10.1016/j.jmb.2005.06.077

Figure 4.

Figure 4

Tail peptide effects on topoisomerase activity. Topoisomerase assay of wild-type Int, W350ter, and A241V-W350ter in the absence or presence of the heptameric C-terminal tail peptide (Tail), and W350G and I353M peptides. Activity was assayed by the conversion of 0.4 μg of pBR327 supercoiled DNA (s.c.) to a relaxed circle (rel.). Reactions were incubated at 25 °C for 15 minutes in the same buffer as the Holliday junction resolutions (Figure 2), except for the presence of EDTA (1 mM), and contained 120 nM of protein. Reactions were quenched with gel loading buffer containing 1% SDS, electrophoresed on 1% (w/v) agarose Tris–acetate–EDTA (pH 8.2) gels, and visualized by staining with ethidium bromide.