Table 1.

Reduced glutathione concentration in rat liver following acetaminophen treatment.

	μmoles rGSH/g liver
Time	Control	150 mg/kg	1500 mg/kg
6h	5.5 ± 0.43	3.8 ± 0.13*	2.3 ± 0.15*
24h	5.2 ± 0.68	5.9 ± 0.02	4.6 ± 0.75

Rats were administered a single acute dose of acetaminophen by gavage at sub-toxic (150 mg/kg) or overtly toxic (1500 mg/kg) doses in 0.5% aqueous methyl cellulose (vehicle control). Liver tissue collected at 6 or 24h post-dosing was analyzed for reduced glutathione (rGSH) content as described in Materials and Methods. Hepatic rGSH concentration is expressed as μmoles/g liver ± standard deviation from 3 animals per group.

^*Statistical difference (p < 0.01) from control group using one-way ANOVA followed by Tukey’s multiple comparison post-hoc test.