Table 2.
Expression of DNA repair genes in rat liver after treatment with an overtly toxic dose (1500 mg/kg) of acetaminophen.
| DNA Repair Gene | Control | 6h | 24h | 48h |
|---|---|---|---|---|
| Ogg1, 8-oxoguanine DNA glycosylase 1 | 3.8 ± 0.8 | 3.9 ± 0.8 | 8.2 ± 2.8* | 10.2 ± 1.5* |
| Mpg, N-methylpurine DNA glycosylase | 6.0 ± 0.4 | 5.2 ± 1.3 | 4.8 ± 2.7 | 4.0 ± 0.8 |
| Ape, purinic/apyrimidinic endonuclease 1 | 23.5 ± 1.4 | 21.6 ± 5.2 | 34.8 ± 2.8* | 30.8 ± 1.7 |
| Pol β, polymerase (DNA directed) β | 13.2 ± 0.9 | 11.9 ± 1.3 | 18.7 ± 1.8* | 15.8 ± 1.2 |
| Pol δ, polymerase (DNA directed) δ | 1.5 ± 0.3 | 1.7 ± 1.7 | 6.0 ± 1.6* | 8.8 ± 2.8* |
| Pcna, proliferating cell nuclear antigen | 20.7 ± 2.1 | 22.5 ± 2.6 | 33.6 ± 7.9 | 50.9 ± 7.6* |
| Parp, poly (ADP-ribose) polymerase | 15.3 ± 0.5 | 21.4 ± 1.3* | 27.6 ± 3.8* | 29.1 ± 1.6* |
| Mgmt, O6-methylguanine DNA ethyltransferase | 29.8 ± 1.3 | 24.1 ± 5.7 | 32.2 ± 6.6 | 32.1 ± 0.1 |
Total RNA was isolated from liver samples and analyzed by RNase protection assay. The results are mean ± standard deviation from 3 animals per group. The relative expression of each gene was normalized to the expression of the housekeeping gene L32. The control is pooled RNA from three biological replicates and then 6, 24, and 48h time points averaged together. The results from animals given a sub-toxic dose of APAP (150 mg/kg) are not presented since they were not significantly different from controls.
Statistical difference (p < 0.05) from control group using one-way ANOVA followed by Tukey’s multiple comparison post-hoc test.