Figure 2.

Treatment of in vitro HIV-1-infected PBMCs with the anti-CD25 IT. PBMCs were infected with HIV-1_JR-CSF and cultured for 6 days in CM with or without 10 nM anti-CD25 IT. After treatment, DNA was extracted, and viral fragments were amplified by PCR. (Upper Left and Middle) The presence of RU5 and RU5-PBS-gag fragments is seen in both treated and untreated cultures. (Upper Right) The presence of full-length viral reverse transcripts is seen in untreated cultures, but not in IT-treated cells. (Lower) β-actin was used as a control for input DNA and amplification. After PHA activation, both treated and untreated PBMCs contained full-length reverse transcripts and produced p24 antigen (data not shown). Serial dilutions of 8E5 cells in PBMCs were amplified to demonstrate that the sensitivity of the assay was >2 viral genomes in 10⁵ cells as reported (16).