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. 1999 Sep 28;96(20):11525–11530. doi: 10.1073/pnas.96.20.11525

Figure 4.

Figure 4

GGPP reverses the effect of simvastatin on TGFβRII expression. (A) Embryonic chicken atrial cells were transfected with pTGFβRII-500/36-Lux plus pCMVβgal. After recovery, cells were incubated for 16 h in media with LPDS plus: lane 1, no additions; lane 2, 10 μM simvastatin; lane 3, 10 μM simvastatin plus 10 μM FPP; lane 4, 10 μM simvastatin plus 10 μM GGPP. Luciferase activity was normalized to β-galactosidase activity. Data are plotted as the mean ±SEM of four independent experiments. (B) Cells were grown in media supplemented with LPDS and treated as described above. Thirty micrograms of crude cell extract were analyzed by immunoblotting for the expression of TGFβRII protein. Data are typical of three similar experiments.