Figure 2.

Binding of IL-18 to SDS/PAGE-purified recombinant proteins. Metal affinity resin-purified recombinant proteins were treated with SDS in the presence or absence of 2-mercaptoethanol and were analyzed by SDS/PAGE. The proteins were transferred to a membrane and were incubated successively with rhIL-18 and an hIL-18 mAb. Bound mAb was detected by chemiluminescence (Left). The membranes then were stripped and incubated with a mAb to the histidine tag (Right). His-tag, 6-histidine tag; other abbreviations are as in Fig. 1.