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Cross-linking of IL-18 to native recombinant proteins. Metal affinity resin-purified recombinant proteins were incubated with rhIL-18 in the presence of bis(sulfosuccinimidyl) suberate and then were analyzed by SDS/PAGE. The proteins were transferred to a membrane and then were incubated with polyclonal Ab to hIL-18. The Ab was detected by chemiluminescence. SA, bovine serum albumin; OA, ovalbumin; other abbreviations are as in Fig. 1.
