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. 1999 Sep 28;96(20):11537–11542. doi: 10.1073/pnas.96.20.11537

Figure 4.

Figure 4

Detection of IL-18 binding to recombinant proteins by using surface plasmon resonance. Metal affinity resin-purified recombinant proteins were individually immobilized on a CM5 chip with the standard amine coupling procedure. Injection of rhIL-18 started at 0 seconds and stopped at 750 seconds. For huIL-18BP, rhIL-18 concentrations used were 0.35, 0.7, 3.5, 7, 10.5, 17.5, 21, and 24.5 nM. For MC54L, rhIL-18 concentrations used were 3.5, 7, 10.5, 14, 17.5, 21, 24.5, and 28 nM. For MC53L, rhIL-18 concentrations used were 7,14, 28, 42, 56, 70, 84, 112, and 140 nM. The colored and black lines are the actual responses in RU and globally fitted curves, respectively. The residual responses, below each set of curves, represent deviations of the actual responses from the fitted curves. The rms deviations were 0.749, 0.369, and 0.236 for huIL-18BP, MC54L, and MC54L, respectively.