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Inhibition of IFN-γ induction. KG-1 cells were incubated with 20 ng/ml TNF-α and either 10 ng/ml of rhIL-18 or rhIL-18 premixed with the indicated amounts of metal affinity resin-purified recombinant protein. After 24 h at 37°C, clarified supernatants were assayed for human IFN-γ.
