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Modulation of M. tuberculosis H37Rv transcript levels during growth in human macrophages as detected by cDNA probes prepared by SCOTS. Bacterial cDNA probes prepared by three rounds of SCOTS (see text) from intracellular bacteria 18 (A), 48 (B), and 110 h (C) after infection were hybridized to PCR-amplified plasmid fragments containing the indicated H37Rv genes (lanes 1–4) or cloned bacterial cDNAs isolated from phagocytized bacilli (48 h).
