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. 1999 Sep 28;96(20):11572–11577. doi: 10.1073/pnas.96.20.11572

Figure 4.

Figure 4

Functional importance of Purα-associated RNA in Tat and Purα effects on LTR activity. U-87MG cells were transfected with 0.5 μg of LTR-luciferase reporter construct in the absence or presence of 10 μg of expression plasmids for Tat, Purα, and the 338-base RNA in the sense or antisense orientation, as indicated at the bottom of the graph. Luciferase activity was determined 36 h after transfection, and the levels of promoter activation by Tat, Purα, and the 338-base RNA, alone or in combination, were determined and graphed as fold activation. In all cases, the final amount of DNA in the transfection mixture was brought to 35 μg with pCMV DNA. The results shown represent the mean of three independent experiments. Bars indicate standard deviation.