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Lack of Dam methylation alters protein secretion. An SDS gel with 8% tricine showing proteins present in culture supernatants from SL1344 (wild type), SV1610 (Dam−), SV1610/pTP166 (Dam−/Dam+), SV4056 (PhoP−), and SV4089 (Dam− PhoP−) strains. Indicated are flagellin (filled arrow), SipA, SipB, and SipC proteins (empty arrows), and three proteins present in supernatants of Dam− strains (arrowheads).
