Table 3.
Kd values characterizing the interaction between the various oligomeric forms of IN during complex formation with different ODNs
| Oligonucleotide | Kd(1)a (µM) | Kd(2)a (µM) | Kd(3)a (µM) | Kd(4)a (µM) | Kd(5)a (µM) |
|---|---|---|---|---|---|
| ss A5 | 2.6 ± 0.4 | 12.0 ± 2.0 | 180 ± 86 | 10.0 ± 2.0 | 120 ± 4 |
| ss GCAGT | 7.1 ± 0.7 | 13.3 ± 1.1 | 340 ± 76 | 24.9 ± 5.6 | 183 ± 16 |
| ds A21 · T21 | 0.040 ± 0.010 | 18.0 ± 4.0 | 250 ± 80 | 17.0 ± 3.0 | 100 ± 40 |
| Ss 19-CA | 0.030 ± 0.015 | 131 ± 34 | 277 ± 41 | 2300 ± 850 | 0.66 ± 0.13 |
| Ss 21-GT | 0.0106 ± 0.01 | 253.6 ± 40 | 206.0 ± 4.0 | 447.0 ± 65.0 | 0.0087 ± 0.0004 |
| ds 19-CA or (19-CA) · (21-COM) | 0.017 ± 0.007 | 133 ± 16 | 127 ± 8 | 15090 ± 469 | 0.010 ± 0.003 |
| ds 21-GT or (21-GT) · (21-COM) | 0.0022 ± 0.001 | 209 ± 83 | 138 ± 7 | 4475 ± 154 | 0.015 ± 0.005 |
aEach Kd value corresponds to a different stage of IN oligomerization according to Model A. The average error in compliance of all calculated parameters with experimental SAXS data corresponding to all mixtures analysed was ∼3–6%.