Table 5.

Relative amounts of different IN monomeric and oligomeric forms in the presence of specific and non-specific ODNs

	Initial concentrations (µM)		Relative amount of different IN forms in equilibriuma (%)
Ligand	E0	S0	E	ES	E2S	E2S2	E4S4	Activationb (%)
ss A5	40.0	10.4	18.7	70.1	0.6	0.7	9.9	(Inhibitor)
	39.0	24.6	8.9	76.5	0.7	0.9	13.0
	34.0	48.9	4.5	79.0	1.2	1.3	14.0
	29.0	83.2	3.8	78.4	1.3	1.5	15.0
ds A21 · T21	40.0	0.17	19.0	69.4	0.7	0.9	10.0	(Inhibitor)
	38.0	0.41	8.9	75.1	0.9	1.1	14.0
	34.0	0.76	5.0	75.9	1.4	1.7	16.0
	29.0	1.30	3.0	75.1	1.6	2.0	18.3
ss GCAGT	40.1	30.8	18.7	66.8	0.5	0.6	9.8	40
	37.9	72.7	9.0	76.5	0.7	0.9	12.9
	34.7	133.3	5.1	77.7	1.2	1.3	14.7
	29.8	228.6	3.0	80.7	1.0	1.1	14.3
ss 19-CA	59.62	39.5	39.00	47.46	8.42	4.85	0.27	225
	55.78	92.1	0.90	84.15	0.32	14.30	0.39
	51.71	169.1	0.24	85.25	0.079	13.56	0.89
	45.05	289.6	0.11	86.60	0.033	12.19	1.09
ss 21-GT	61.78	31.3	53.60	37.4	4.87	4.17	0.024	100
	53.77	73.0	0.093	82.0	0.017	17.50	0.370
	49.70	143.0	0.012	83.1	0.002	16.58	0.300
	43.30	229.0	0.005	84.76	0.007	15.00	0.220
ds 19-CA	64.20	14.0	79.38	15.69	3.82	3.82	∼0.0	250c
	57.15	32.6	49.35	49.35	5.30	5.30	0.005
	48.10	59.9	0.89	77.83	0.16	21.10	0.050
	41.85	102.6	0.036	80.34	0.0055	19.60	0.024
ds 21-GT	62.92	15.1	77.23	15.76	5.78	1.23	∼0.0	210c
	55.47	35.3	43.80	41.20	7.53	7.43	∼0.0
	47.67	64.7	0.13	77.37	0.036	22.47	0.0016
	41.54	110.8	0.014	79.38	0.0034	20.60	0.0012

^aThe average error in compliance of all calculated parameters with experimental SAXS data corresponding to all mixtures analysed was ∼3–6%.

^bData on IN activation are taken from (21).

^cIN activation were determined in this article according to (21).