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. 2006 Dec 20;35(3):755–770. doi: 10.1093/nar/gkl1088

Figure 1.

Figure 1

Complementation of pea RPA32 and RPA70 genes in the ts mutant strains of yeast. Pea RPA32 and RPA70 genes were cloned in frame with yeast pGAD vector and transformed in the ts mutant of yeast for RPA32 (HMY345) and RPA70 (W303-1a/rfa1-t6) genes respectively. The transformants were selected on synthetic yeast media lacking leucine at 25°C. Panel (a) shows independent colonies of the yeast ts mutant strain of RPA32 transformed either with pGAD vector [(i) and (ii)] or with pGAD-PsRPA32 construct [(iii) and (iv)] and grown at 25°C (permissive) and 37°C (restrictive). Similarly, panel (b) shows the yeast ts mutant strain of RPA70 transformed either with pGAD vector [(i) and (ii)] or with pGAD-PsRPA70 construct [(iii) and (iv)], grown at 25° and 37°C.